The purpose of the Terms is to establish the rights and obligations of Psomagen and Client with respect to Psomagen's service or product as requested or ordered by Client. Psomagen and Client shall faithfully perform their duties as specified in these Terms.
Unless specified otherwise in these Terms, the following terms have the meanings set forth herein.
These Terms shall become effective as of the date when Client places an order and shall remain in full force and effect till the completion date unless agreed otherwise.
|Service name||Analysis sample||data|
|CES||3 months||3 years|
|NGS||3 months||3 months|
|Clinical||3 months||3 months|
After placing an order, Client may request and receive additional technical support or consulting service from Psomagen. In the event that such support or service incur any additional costs, Psomagen shall notify and discuss with the client in advance.
The “Gold Standard of Sequencing” is offered as a service with fast turnaround time, quality of results, and competitive prices.
Our automated systems allow rapid and accurate processing.
Samples can be submitted in premixed state or template/primer separately for single tube, plate, or colonies.
Standard sequencing is a service that sequences PCR products and plasmid DNA requested by customers.
Psomagen provides quicker and more accurate services based on the Capillary Electrophoresis Sequencing (CES)
automation system and extensive experience.
After the results are delivered, an expert in the sequencing field is ready to provide help until the follow-up.
Standard sequencing can be performed with single tubes and 96-well plates.
Psomagen offers DNA sequencing for difficult templates with high GC or GT, unusual secondary structure or hairpin structures.
With over 20 years of experiences, we have optimized our protocol to yield successful sequencing data for these difficult templates.
Psomagen offers primer walking service to confirm the sequence integrity of your clone or PCR products.
We can perform end sequencing with the primers that you provide or use the reference sequence, if applicable, and design internal primers to fill in the gap.
EZ-Seq is pre-paid DNA sequencing services devised to eliminate complicated ordering process.
Instead of filling out an order sheet for sample and primer information, you are only asked to enter the number of reactions you would like to request.
You will be given a barcoded label per each tube or plate which will serve as an identifier for your sample(s).
Samples must be in premixed state and in-house universal primers cannot be used for Ez-Seq.
Barcoded label will be provided upon receiving payment.
How to use EZ-Seq
We have been dedicating to provide genomic solutions to researchers in all over the world with its advanced DNA sequencing technology by using ABI 3730XLs. Thanks to over 20 years long know-how and large genomics facility, we are able to provide our sequencing service from small scale sequencing reactions to large sequencing projects, we are ready to serve you with our pleasure.
Psomagen performs PCR service with the template and the primer set to be provided by customer.
PCR condition, primer Tm value, predicted product size, primer concentration, and template concentration are required.
We conduct PCR optimization process for each primer set for high quality of PCR products from gDNA.
Primer design and synthesis can also be done with extra charge
16S/18S/28S rRNA and ITS region full sequencing is a service that analyzes sequences
by amplifying ribosomal RNA genes of bacteria and fungi (filamentous fungus and yeast)
and confirming homology of target microorganisms using an rRNA database (NCBI).
Psomagen controls and performs all processes from gDNA extraction of bacteria
and fungi (filamentous fungus and yeast) to PCR amplification, purification, sequencing, and BI report.
For bacteria, PCR of 16S rRNA genes is performed using 27F and 1492R primers, and sequencing is conducted using 785F
and 907R primers, which are the inter-primers, to identify bacteria. If the customer requests,
a primer can be additionally selected/changed and about 1,350 bp or longer sequences can be provided.
The default 2rxn (785F, 907R) is selected to allow more primer runs if desired.
1,600 bp or higher results are guaranteed by the sequencing of the 18S rRNA region.
500 bp or longer results are guaranteed by the sequencing of ITS region.
1,300bp or longer results are guaranteed by the sequencing of 28S rRNA gene (D1/D2/D3 region).
Fragment Analysis encompasses a wide variety of genotyping, DNA profiling, and mutation detection techniques for a/an medical, environmental, and agricultural research.
Psomagen Corp. provides the Fragment Analysis based on our accumulated experiences and knowledge in genomics.
In general, this service is used to only check amplified fragment sizes.
As PCR amplification is not available through this service, fluorescent-labeled PCR products should be supplied as Microsatellite Analysis (VNTRs) samples.
|Internal Standard Size Marker
(Maximum detection size)
- Utilizing the advanced technology from Oxford Nanopore Technologies, Whole Plasmid Seq enables entire plasmid to be sequenced without the need for any primers
- Without any biased from PCR reactions, Whole Plasmid Seq can deliver more accurate results and faster turnaround time than Primer Walking
- 16 to 24 hour turnaround time
- Results delivered in FASTA (consensus data) FASTQ (raw data) format
- 99% + Accuracy (Q20+)
- New and improved V14 Chemistry and updated R10.4.1 nanopore to provide the highest accuracy
Sample Type: Plasmid DNA
Length: Up to 30 kb (If larger than 30 kb, contact us)
Concentration: 50 ng/µl
Volume: Minimum 15 µl
Purity (A260/280): 1.8 – 2.0
Samples and primers can be submitted in 1.5 ml tubes, strip tubes or 96 well PCR plates.
As for the DNA samples in water or TE, bacterial cells in agar-stab, or agar plate culture, temperature control is not necessary.
Samples are stable for a few days at room temperature.
The quality of DNA is the single most important factor in obtaining high quality sequence data.
To ensure proper concentration, please check your samples by electrophoresis prior to shipping. There should be a single clear band of the appropriate size when loading 1 µl of the sample on a 1% agarose gel with EtBr. Smearing or multi-bands can be causal factors in sequencing failures. Using UV absorbance to quantify DNA may provide inaccurate measurements of target DNA concentration.
DNA should be dissolved in Nuclease-Free distilled water. Nuclease-Free super low EDTA TE buffer (10mM Tris-HCI, pH8.0, 0.01mM EDTA) can be used but with caution. If the TE buffer with EDTA (concentration of 0.1-1Mm) is used for dissolving DNA, EDTA take up free magnesium ions, which reduces DNA polymerase activity resulting in sequencing reaction failure.
|Sample Type/Format||DNA size||Concentration||Volume|
|Plasmid||4 ~ 8kb||80 ~ 150 ng/µl||10 µl per reaction|
|8 ~ 15kb||150 ~ 200 ng/µl|
|PCR Product||Less than 300bp||10 ~ 20 ng/µl|
|300bp ~ 700bp||20 ~ 30 ng/µl|
|700bp ~ 1kb||30 ~ 50 ng/µl|
|1kb ~ 4kb||50 ~ 100 ng/µl|
|Over 4kb||See Plasmid|
|BAC||40 ~ 200kb||500 ~ 1000 ng/µl||15 µl per reaction|
|gDNA||-||30 ~ 50 ng/µl||15 µl per sample|
|Whole Plasmid Sequencing||Up to 30kb||50 ng/µl||15 µl per sample|
|Premix||Plasmid||-||100 ng/µl||5 µl sample + 5 µl primer|
|Purified PCR Product||-||50 ng/µl|
|Sample Type/Format||DNA size||Concentration||Volume|
|Primer for regular sequencing||18 ~ 27 mer(Tm 55℃ - 60℃)||2 ~ 5 pmol/µl||10 µl per reaction|
|Primer for BAC sequencing||100 pmol/µl|
We provide the shipping protocol to make all users of Psomagen’s life easier!
Air waybills and commercial invoices will be provided to you in a few clicks through this protocol.
As your account carries all of your shipping information, sender information will be automatically submitted to you.
You can also save up to three different addresses for your future orders.
You are able to print the waybill, the commercial invoice and the letter of acceptance with all shipping details already entered according to the waybill information when you click the Continue button.
When all three steps have been completed, call your local FedEx or DHL to request for pickup!
Don’t worried about the safe arrival of your samples after dispatching,
we have the monitoring system for the packages that you send us to promptly resolve all the problems happened during shipping.
Most of sequencing failure is caused by sample condition or the specificity of composition, in this case there is no possibility of improvement through any other approach use, and therefore it is not the case of reanalysis Psomagen sequencing policy. The patterns in problems have been summarized to help user's understanding in sequencing data.
The objective of reanalysis in Psomagen sequencing service is to confirm the possibility of machine' error, operator's mishandling, and only reanalysis is carried out in the case of that improvement is expected. Therefore, sending a sample from a new batch is not considered the subject for reanalysis even though it is the same name. The following failures caused by template preparation or composition should be fully paid for the analysis service.
* The primer binding site might be damaged. We would like to recommend that you try to use an alternative primer.
This agreement is a contract between you and Psomagen, Inc. (hereafter, Psomagen) and applies to Psomagen’s services usage in whole. You shall read, agree with and accept all of the terms and conditions contained in this agreement.
This agreement is to comply with the law of electric communication enterprise and an Enforcement Ordinance in the United States of America on the utilization stipulation and procedure of all the related services provided by Psomagen, Inc.
Service defines that it furnishes DNA sequencing and other additional information through https://order.psomagen.com to be provided by Psomagen, Inc. hereunder.
Psomagen can delete the notice posted by users for the following without any pre-notice.
All disputes, controversies or differences which may arise out of or in connection with the Contract or for the breach thereof shall be finally settled by arbitration in the USA in accordance with the Commercial Arbitration Rules of the USA.
The Contract shall be governed and interpreted by the laws of the USA.
Unless otherwise provided herein, Supplier shall not be liable for any consequential, direct or indirect damages, related to free service except for the damages caused by willful misconduct.
We are not liable for damage or losses to any of one’s standard sequencing result files which are stored in Psomagen’s server resulting from participation in or accessing or downloading file or data in connection with the Service.
We reserve the right, in our sole discretion, to cancel or suspend the Service should a virus, bugs, or other causes beyond our control corrupt the administration, security or proper operation of the Service.
You shall pay liquidated damages, not as a penalty, to Psomagen in an amount of 10% of the total amount of the delayed payment beyond the due date.